Conclusion: Our study emphasized on rapid identification of NTM by Genome sequencing within 8-10 hours of isolation which was advantageous over the conventional method. The in vitro susceptibility of 30 NTM isolates showed sensitivity to AMK and TOB (29), AZM (27), CFX, MFX, IMP, CLR, GAT (26), OFX, DOX (25) and NFX (24). Results: Among 30 clinical isolates, majority of NTM were identified as Mycobacterium abscessus, followed by M. isolates were put up for Amikacin (AMK), Ciprofloxacin (CFX), Moxifloxacin (MFX),Norfloxacin (NFX), Ofloxacin (OFX), Clarithromycin (CLR), Azithromycin (AZM), Imipenum (IMP), Tobramycin (TOB), Gatifloxacin (GAT) and Doxyclyclin (DOX) by disc diffusion method.
Materials and Methods: A total of 30 NTM isolates from 1416 clinical specimens were subjected to genome sequencing targeting hsp65 gene for the identification of NTM. Laboratory diagnosis of NTM is crucial for the proper management of the patients. A detailed interaction of these pathogens in theīlood need to be further characterized to explore the targeted therapiesīackground: Non-tuberculous Mycobacteria (NTM) are ubiquitous in the environment and gaining attention due to emerging outbreaks. RRNA mutation conferring resistance to spectinomycin.Ĭonclusion: The detection of normal respiratory flora (infrequent infectiousĪgent) in the patient’s blood samples could be an alarming With antibiotics resistance database revealed the presence of 16S Submitted in the genbank (accession numbers: MN394393, Similarity with Neisseria polysaccharea and the sequences were BLAST analysis with 16S rRNA showed a high The antibiogram also showed resistance to Vancomycin, PenicillinĪnd co-trimoxazole. However, the isolate was not identified by the Vitek2 and MALDI. The smear morphology resembled Neisseria meningitidis. Results: All three blood cultures initiated upon admission were With Bioinformatics methods such as NCBI-BLAST and The sequence of the PCR products were validated 16S bacterial rRNA gene wasĪmplified by the polymerase chain reaction (PCR) followed by Was performed by conventional biochemical tests and commerciallyĪvailable Vitek 2 compact. Showed positive signal and the subsequent isolation of gram Methods: Three consecutive blood cultures were performed Study was conducted to ascertain the causative agent of the acuteįebrile illness (AFI) with fulminant sepsis and disseminated intravascular In the blood has been associated with severe infections. Frequently, the presence of these bacteria Background: Nongonococcal, Nonmeningococcal Neisseriae are part